Explaining predicted cross-reactivity

How does BenchSci calculate predicted cross-reactivity on product pages?

Although antibodies can be highly specific, similarities in epitope (the region of an antigen that an antibody recognizes) between homologs or otherwise similar targets can lead to (un)desirable cross-reactivity. Our cross-reactivity analysis can be extremely valuable for identifying new uses or targets for an antibody in species that were not explicitly tested for by the vendor. 

We’ve addressed this by conducting in-house, in-silico cross-reactivity analysis for over 400k products where the epitope sequence was available and calculating the compatibility of the intended epitope with other targets. This analysis is found under the Reactivity heading for analyzed antibody products.

The percentage displayed is the percent sequence similarity between the epitope sequence and the analogous sequence in all isoforms across twelve model organisms. We show high and low sequence similarity results since the epitope may be truncated or deleted in some isoforms.