Could you explain your predicted cross-reactivity analysis?

This article explores the ins and outs of BenchSci's predicted cross-reactivity analysis found on certain product pages.

Some antibodies can cross-react with similar epitopes on different proteins.

To address this, we've conducted a predicted cross-reactivity analysis, in-house, in-silico, for over 400k products where the epitope sequence was available.

Transcript:

Some antibodies can cross-react with similar epitopes on different proteins. These can be different proteins in the same species, which determines antibody specificity, or similar proteins in different species.

To address this, BenchSci has conducted a predicted reactivity analysis in-silico for over 400 thousand products where the epitope sequence was available. When available, you can find this analysis in the product page under the ‘Reactivity’ heading.

What we display here is the percent sequence similarity to the epitope sequence for all isoforms within twelve model organisms. We display both high and low sequence similarity results, since it’s possible that the epitope is absent or truncated in an isoform.

If you click on a result, you can click here to be linked out directly to the UniProt ID page for that isoform.

Using this analysis, you can determine whether the epitope is present in the isoform you’re working with (for example, like it is here in an isoform of the vendor recommended species).

You can also make predictions about specificity of the antibody, or whether it’s likely to detect other isoforms of the protein in the species you’re working with.

And you can also potentially repurpose an existing antibody for use in a new species, even if it’s not vendor recommended.

Vendors don’t have the capacity to test their product in every species, which makes this analysis incredibly valuable.