Filtering for different Cas nucleases and PAM sequences

A wide selection of Cas nucleases are available under the Cas Type filter

One of the many draws and advantages of using the CRISPR/Cas nuclease system for gene manipulation is its flexibility and applicability across a wide array of systems, organisms, and experiments. Thus, in addition to filters for Application (e.g. CRISPR knockdown, knockin, etc.), you can also filter for specific Cas Types to find the one with the desired nuclease activity and PAM (protospacer adjacent motif) sequence. Find the Cas Types filter under Cas Nuclease specs. Your choices include:

Cas Type

PAM

Notes

Cas9 (aka SpCas9)

NGG

Streptococcus pyogenes

Blunt-ended dsDNA break. gRNA spacer length ~18-24 nt.

Cas9 null (aka SpCas9 null)

NGG

Streptococcus pyogenes

Engineered not to cut DNA. May be used for targeting.

Cas9 nickase

NGG

Streptococcus pyogenes

Creates a single stranded cut. May be used with two adjacent sgRNAs to reduce off-target effects.

saCas9

NNGRR(N)

Staphylococcus aureus

Blunt-ended dsDNA break. Similar to SpCas9 in use.

saCas9 null

NNGRR(N)

Staphylococcus aureus

Engineered not to cut DNA.

Cas12a (aka Cpf1)

TTTV

May target and cleave single stranded DNA. Cas12a may also leave 5’ overhang after cleavage.

If there are additional Cas nuclease Types you would like to see on the platform, let us know. We’d love to chat about it.