The different CRISPR experiments include knockout, knockin, activation, interference, screen, methylation, and more
To help you survey the literature and product catalogs for CRISPR reagents, we’ve built four of the
most common CRISPR experiment types into our Application filter. When you set the Product Type filter to CRISPR gRNA or CRISPR Cas Nuclease, you’ll be presented with a growing list of Application types. The most common among them are CRISPR Knockout, CRISPR Activation, CRISPR Knockin, CRISPR Interference, CRISPR Screen, Tagging, Methylation, Demethylation, and more.
CRISPR Knockout (CRISPR KO) - guide RNA (gRNA) disrupts the targeted gene by way of a Cas nuclease-mediated double stranded break (or single stranded nicks) that is erroneously repaired by non-homologous end joining (NHEJ).
CRISPR Knockin (CRISPR KI) - a gene targeted by gRNA is modified by adding sequences (e.g. disease SNPs) that will then be used as a template for repairing the double stranded break (or single stranded nicks) through the homology-directed repair (HDR) mechanism.
CRISPR Activation/Interference (CRISPRa/i) - A deactivated Cas nuclease (e.g. dCas9 or Cas9 null) is used for its precise targeting which enables gene activation in conjunction with fused transcriptional activators, or inhibition by sterically blocking transcription.
CRISPR Screen - Typically, a genetic loss-of-function experiment across an organism's whole genome coupled with selective pressure and then sequencing (e.g. next generation sequencing). It's an approach for identifying novel protein functions.
CRISPR Tagging - CRISPR technology can be used to tag proteins that are expressed in their normal chromosomal context.
When you select more than one application simultaneously, we will return products (and figures that used those reagents!) that are indicated for or used for both types of experiments. Read more about some of the principles of search logic on BenchSci here.
If there are CRISPR applications that are important to you that we’re missing, let us know and we’d love to talk to you about them.