Guide to Filters for CRISPR Reagents

Our filters are key features that empower scientists to quickly focus their search for CRISPR reagents from millions of figures and products in our database to help them design better, more successful experiments. 

If there are any types of filters that will help make your search easier and help define your experimental context, please let us know at support@benchsci.com! To help you survey the literature and product catalogs for CRISPR reagents, we’ve also built some of the most common CRISPR experiment types into our Application filter.  

How to apply filters

  1. Simply type in search criteria into the search bar (i.e. target protein/gene or Cas nuclease specs)
    • More information about how to build more complex searches in the search bar can be found here!
  2. Click the filter on the left side to add it to your search criteria (you will see it update in the search bar)
    • Some filters include an option to Exclude certain selections (ex. if you want to exclude a particular Selectable Marker)
    • Some filters also include checkboxes to include more than one selection at a time
    • Note: The order in which the filters are presented here may not necessarily reflect how they appear on the platform, as some users may see an alternative recommended filter view

Search logic on the platform

  • During your search, you may want to start combining filters of different types (e.g. Application, Organism Tested...) or multiple instances of the same filter (e.g. Application: Western Blot, Immunohistochemistry)
  • The search logic on BenchSci—that is the use of AND, OR between search terms—aims to be intuitive
    • Search logic is described below under each filter type!

Filter type: Application

  • This filter allows you to narrow down your results based on what type of experiment the product may be used in
    • Based on vendor recommendations or how it has been used in experimental applications in publications
  • Selections include: western blot, immunofluorescence, immunohistochemistry, flow cytometry, knockdown etc.
    • You can also search with common abbreviations/aliases like WB, IF, IHC or flow in the search bar
  • Search logic for multiple selections
    • Product results: AND (all products must have been used with that context for all selections)
    • Figure results: OR (all figure results must contain at least one of the selections)

Filter type: Figure Usage Data

  • These filters help narrow down your search results based on how the product was used in literature
  • Search logic for multiple selections
    • Product results: AND (all products must have been used with that context for all selections)
    • Figure results: OR (all figure results must contain at least one of the selections)

DescriptionExample
Organism Tested
Model or organism in which the experiment was conducted or from which cells or tissues were obtained

This filter also shows a list of aliases/synonyms (ex. homo sapiens will be grouped under human)
Human
Mouse
Drosophila

Tissue Used
Type of tissue that experiments using the product were performed in
Liver
Lung
Cell Type Used
Cell type that experiments using the product were performed in
Epithelial
Neuron
Cell Line Used
Specific names of cell lines that experiments using the product were performed in
HEK293
HeLa cells
Disease
Disease context that experiments using the product were associated with
Breast cancer
Chronic kidney disease

Filter type: Supplier

  • These filters narrow down the results based on information about the vendors themselves
  • Hover over a selection when you open a filter to add Exclude to remove vendors from your search
  • Search logic for multiple selections (Company): OR (the product or figure results need to match at least one of the companies)

Description
Availability
This filter will hide discontinued or unavailable products—the default option is that all products will be shown
Company
The vendor that sells the product. You can choose preferred vendors, or exclude vendors you want to avoid

Filter type: gRNA Specs

  • These filters narrow down the results based on product information that is provided by the vendor
  • Hover over a selection when you open the filter to add Exclude to remove certain results from your search
  • Search logic for multiple selections for both product and figure results
    • AND: Target Species, Promoter, Selectable Marker
    • OR: Cas Compatibility, Target Location, Vector Format, Reporter

DescriptionExample
gRNA Type
The type of guide RNA product
sgRNA (single guide RNA)
crRNA (CRISPR RNA)
Target Species
The species of the target gene/protein that the gRNA is designed for
Human
Mouse
Cas Compatibility
Cas nuclease that the gRNA is designed to target
Cas9
Cas9 nickase
Sequence
If you only want to show gRNA products with a known sequence

Target Location
Genomic region of a gene that the gRNA targets
Exon 4
Exon 5
Vector Format
Vector format for the gRNA product (if it comes as a vector)
Lentiviral particles
AAV particles
Promoter
The promoter in the expression vector (if it comes as a vector)
CMV promoter
Tet promoter
Reporter
The reporter in the expression vector (if it comes as a vector)
eGFP
mCherry
Selectable Marker
The selectable marker in the expression vector (if it comes as a vector)
Puromycin
Zeocin

Filter type: Cas Nuclease Specs

  • These filters narrow down the results based on product information that is provided by the vendor
  • Hover over a selection when you open the filter to add Exclude to remove certain results from your search
  • Search logic for multiple selections for both product and figure results
    • AND: Modification, Purity, Formulation, Promoter, Selectable Marker
    • OR: Cas Type, Vector Format, Reporter, Purification Method

DescriptionExample
Cas Nuclease Format
The format of the Cas nuclease product
Vector
Protein
mRNA
Cas Type
The type of Cas nuclease — can be different species or mutants
Cas9
Cas9 nickase
Modification
Modification of the Cas nuclease, can be a tag or an activation/repressive domain
His tag
Flag tag
Purity
Purity of the product
>95%
>80%
Purification Method
Method used to purify the product
HPLC purified
Formulation
Formulation of the product
Glycerol
Lyophilized
Vector Format
Vector format for the Cas nuclease product (if it comes as a vector)
Plasmid
Lentiviral particles
Promoter
The promoter in the expression vector (if it comes as a vector)
CMV promoter
T7 promoter
Reporter
The reporter in the expression vector (if it comes as a vector)
GFP
RFP
Selectable Marker
The selectable marker in the expression vector (if it comes as a vector)
Puromycin
Neomycin