How does "immunostaining" differ from IHC, IF, Flow cytometry, etc.

Immunostaining is a broad term used on BenchSci for when it isn't clear which specific antibody-based technique was used in the literature

In the literature, immunostaining is typically used as an umbrella term for experiments that use antibodies to detect your protein of interest. When papers mention immunostaining, they could be referring to immunohistochemistry, immunofluorescence, flow cytometry, and more. In early versions of BenchSci, if paper authors didn’t give more details than just immunostaining, that’s how those data were sorted in our database. 

We’ve since trained our image recognition machine learning technology to more precisely interpret and reclassify the immunostaining figures and label them with a more specific and meaningful technique (e.g. IHC, IF, FCy) when possible. This way, you get you the most relevant figures and antibodies for your experiment. This is just another way BenchSci is empowering scientists to run better experiments.

Please check out our Guide to Filters for Antibodies to learn more about our filters!