Do you have In-Cell Westerns on BenchSci?

Yes! In-cell westerns are now on BenchSci! You can find them as an option in the Kits filter under Antibody Specs.

The in-cell western has a few different names, including in-cell ELISA, cytoblot, and near-infrared immunocytoblot (see Stockwell, Haggarty, & Schreiber). The goal of the in-cell western is to quantify the amount of a target protein (or post-translational modification of a target) in a cultured cell. The in-cell western requires comparatively little processing and may, therefore, be more quantitative. Adherent or suspension cells are cultured in a microplate, fixed and permeabilized, and then treated with up to two primary antibodies. This is followed by incubation with secondary antibodies — ideally conjugated to a near-infrared fluorophore (see Chen, et al.) — imaging, and then a cell labelling dye is added if normalizing to the cell number. 

 

Start to finish, it’s estimated to take ≤ 5 hrs for 96+ samples.